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目的探讨同源盒基因(HOX)B在多发性骨髓瘤(MM)中的作用。方法用免疫组化法、实时RT-PCR技术、TUNEL法对裸鼠组织细胞中的HOXB基因表达水平进行检测,检测结果经过内参基因β-actin校正后,按2-ΔΔCt法进行计算。得到各组HOXB相对表达量F值,以对照组为参照,HOXB mRNA相对表达率(%)=100%×(其他组mRNA相对表达量F值/对照组mRNA相对表达量F值),计算各组HOXB3 mRNA、HOXB4 mRNA和HOXB7 mRNA相对表达水平。结果在造模实验中,30只大鼠中死亡1只,造模失败2只,27只纳入观察组。观察组HOXB3、HOXB4、HOXB7 mRNA相对表达水平均高于对照组(P<0.05)。而免疫组化法则发现观察组的阳性率远远高于对照组。结论 HOXB基因参与MM形成和发展,在其中扮演了较为重要的角色。
Objective To investigate the role of homeobox gene (HOX) B in multiple myeloma (MM). Methods The expression of HOXB gene in nude mice cells was detected by immunohistochemistry, real-time RT-PCR and TUNEL. The results were corrected by β-actin and calculated by 2-ΔΔCt method. The relative expression of HOXB in each group was obtained. The relative expression rate of HOXB mRNA (%) = 100% × (the relative expression of F mRNA in other groups / the relative expression of mRNA in control group) was calculated based on the control group Group HOXB3 mRNA, HOXB4 mRNA and HOXB7 mRNA relative expression levels. Results In the modeling experiment, one of 30 rats died, two failed to establish model, and 27 rats were included in the observation group. The relative expression levels of HOXB3, HOXB4 and HOXB7 in observation group were higher than those in control group (P <0.05). The immunohistochemical method found that the positive rate of the observation group is much higher than the control group. Conclusion The HOXB gene plays a more important role in the formation and development of MM.