目的:研究藤黄酸在大鼠胆汁中代谢物。方法:应用LCMS-IT-TOF和合成的标准品对代谢物进行研究。结果:藤黄酸在大鼠胆汁中代谢成三个产物,M1,M2和M3。M1的结构推测为3,4-二氢藤黄酸,M2和M3分别确证为10-羟基藤黄酸和9,10-环氧藤黄酸。结论:藤黄酸在大鼠胆汁中主要存在3个代谢产物。代谢物M1是首次被报道的,代谢物M2和M3的结构通过将它们与标准品进行比对而得到确证。 Objective: To study the metabolites of gambogic acid in rat bile. Methods: Metabolites were studied using LCMS-IT-TOF and synthetic standards. Results: Gambogic acid was metabolized into three products in rat bile, M1, M2, and M3. The structure of M1 was presumed to be 3,4-dihydrogambogic acid, and M2 and M3 were confirmed to be 10-hydroxygamboric acid and 9,10-epoxygambogic acid, respectively. Conclusion: Gambogic acid mainly exists in three metabolites in rat bile. The metabolite M1 was first reported, and the structures of the metabolites M2 and M3 were confirmed by comparing them with standards.