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利用多细胞球体 ,探讨体外培养条件下肿瘤坏死因子 (TNFα)对肺癌细胞增生能力的影响。发现TNF( 1 0 4U/mL)与人肺癌细胞系A5 4 9球体共同培养后对A5 4 9细胞球体生长有一定的抑制作用 (P <0 .0 5 )。TNF与A5 4 9单层细胞作用后 ,观察细胞生长曲线的变化 ,计算细胞倍增时间 ,测定细胞集落形成率。发现TNF可改变A5 4 9细胞的生长曲线 ,使细胞倍增时间由 37h延长至 4 3h ,集落形成率由 5 2 %降低为 1 5 %。结果表明TNF对增生性肺癌干细胞有一定的抑制作用。
Using multicellular spheroids, the effects of tumor necrosis factor (TNFα) on the proliferation of lung cancer cells in vitro were investigated. It was found that the co-culture of TNF (104U / mL) and human lung cancer cell line A549 spheroids inhibited the growth of A549 cells to a certain extent (P <0.05). TNF and A549 monolayer cells after the changes in cell growth curve was observed to calculate cell doubling time to measure the rate of cell colony formation. TNF was found to change the growth curve of A549 cells, the doubling time of cells was prolonged from 37h to 43h, and the colony formation rate was reduced from 52% to 15%. The results show that TNF on the proliferation of lung cancer stem cells have a certain inhibitory effect.