目的:探讨Rg_1对神经系统作用的机制。方法:采用Northern和Western印迹分析法,检测了Rg_1处理前后大鼠海马组织的c-fos基因和蛋白的表达。结果:老年鼠c-fos基因和蛋白的表达明显低于青年鼠,但给Rg_1后老年鼠和青年鼠均呈现显著性增强效应。此外,Rg_1还明显增加青年鼠和老年鼠海马组织的cAMP含量。结论:Rg_1升高cAMP水平及促进c-fos基因和蛋白的表达有助于阐明其促智和抗衰老作用的机制。 Objective: To explore the mechanism of Rg_1 on the nervous system. METHODS: The expression of c-fos gene and protein in rat hippocampus before and after Rg_1 treatment was detected by Northern and Western blot. RESULTS: The expression of c-fos gene and protein in aged mice was significantly lower than that in young rats, but significant effects were observed in Rg_1 post-aged and young rats. In addition, Rg_1 also significantly increased cAMP levels in hippocampal tissue of young and old rats. Conclusion: Rg_1 increases cAMP level and promotes the expression of c-fos gene and protein, which helps elucidate the mechanism of its nootropic and anti-aging effects.