目的:探究成体大鼠骨髓中神经嵴源细胞的获取,及对受损周围神经的修复作用。方法:富集骨髓神经嵴源细胞间充质球,分离单克隆球,检测长期增殖能力和神经嵴标志的表达,检测向施旺细胞定向分化的能力和体外成髓鞘潜能。体外构建骨髓神经嵴源细胞为支持细胞的组织工程化神经移植物,桥接大鼠10 mm坐骨神经缺损,12周后进行行为学分析、电生理检测、荧光金逆行示踪试验、肌肉和神经的组织学观察。体外收集骨髓神经嵴源细胞的条件培养基,处理氧糖剥夺损伤的大鼠 OBJECTIVE: To investigate the acquisition of neural crest cells in the adult rat bone marrow and the repair of damaged peripheral nerves. Methods: Mesenchymal spheres of bone marrow neural crest were enriched, monoclonal antibodies were isolated, and the long term proliferative ability and the expression of neural crest markers were detected. The ability of directional differentiation into Schwann cells and the myelin potential in vitro were detected. In vitro, bone marrow neural crest cells were used as tissue-engineered nerve grafts to support cells, bridging the 10 mm sciatic nerve defect in rats. Behavioral analysis was performed after 12 weeks, electrophysiological examination, retrograde tracing with fluorescent gold, muscle and nerve tissue Learn to observe. Conditioned media of bone marrow neural crest cells were collected in vitro, rats treated with oxygen glucose deprivation