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目的:观察西洋参茎叶皂苷(Panax quinquefolius saponins,PQS)对大鼠心肌缺血再灌注损伤的保护作用,并探讨其作用机制。方法:大鼠50只随机分5组,每组10只,即假手术组、模型组、PQS低、高剂量组及尼莫地平组。假手术组和模型组ig给予生理盐水,给药组ig给予PQS(100,300 mg·kg-1)和尼莫地平(21.6 mg·kg-1),每日1次,连续给药15 d后,除假手术组外,手术结扎冠脉建立心肌缺血再灌注模型。观察PQS对大鼠心脏功能及心肌梗死面积的影响;检测PQS对血清肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)及心肌组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平的影响。结果:模型组左室收缩压(LVSP)降低,左室舒张末压(LVEDP)升高,心肌梗死面积增加,血清中CPK,LDH活性及MDA,TNF-α和IL-6含量升高,SOD和GSH-Px活性降低,与假手术组比较均具有显著统计学差异(P<0.01);与模型组比较,PQS和尼莫地平均可以使LVSP升高,LVEDP降低,减少心肌梗死面积,降低血清中CPK,LDH活性及MDA,TNF-α和IL-6含量,升高SOD和GSH-Px活性,均具有统计学差异(P<0.01,P<0.05)。结论:PQS预处理能够有效的减弱心肌缺血再灌注引起的损伤,该作用与抑制心肌缺血再灌注引起的活性氧增加及减少炎症反应的发生有关。
Objective: To observe the protective effect of panax quinquefolius saponins (PQS) on myocardial ischemia-reperfusion injury in rats and its mechanism. Methods: Fifty rats were randomly divided into 5 groups (10 rats in each group), namely sham operation group, model group, PQS low, high dose group and nimodipine group. The rats in the sham-operation group and model group were given ig with normal saline. PQS (100,300 mg · kg-1) and nimodipine (21.6 mg · kg-1) In addition to the sham operation group, myocardial ischemia-reperfusion model was established by ligating the coronary artery. To observe the effects of PQS on cardiac function and myocardial infarct size in rats; To detect the effect of PQS on serum creatine phosphokinase (CPK), lactate dehydrogenase (LDH), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and myocardial tissue superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) levels. Results: The left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) increased, myocardial infarct size increased, serum CPK, LDH activity and the contents of MDA, TNF-α and IL-6 increased (P <0.01). Compared with the model group, both PQS and nimodipine could increase the LVSP, decrease the LVEDP, reduce the area of myocardial infarction and decrease the myocardial infarct size Serum levels of CPK and LDH, MDA, TNF-α and IL-6, and activities of SOD and GSH-Px increased significantly (P <0.01, P <0.05). Conclusion: PQS preconditioning can effectively attenuate myocardial ischemia-reperfusion injury, which is related to inhibiting the increase of reactive oxygen species (ROS) induced by myocardial ischemia-reperfusion and reducing the incidence of inflammatory reaction.