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目的研究4 a亚型猪戊型肝炎病毒(HEV)对猕猴的致病性。方法用1株从广西壮族自治区农村地区幼猪粪便中分离并已经基因测序确定为4 a亚型的猪HEV静脉注射感染猕猴,通过检测粪便HEV RNA、血中HEVRNA、血清丙氨酸氨基转移酶(ALT)水平、HEV抗体及肝组织分别进行病理学和电镜检查确定HEV致病性。结果猪HEV株实验感染猕猴后,出现粪便排毒、病毒血症、ALT升高及HEV抗体阳转,活检肝组织切片呈现典型的急性病毒性肝炎的病理变化,电镜下观察到肝细胞中散在有大量病毒样颗粒。结论广西4 a亚型猪HEV能够跨物种感染猕猴并引起戊型肝炎。
Objective To study the pathogenicity of 4-HE subtype swine hepatitis E virus (HEV) to rhesus monkeys. Methods One pig was infected with pig HEV RNA isolated from excrement of piglets in rural areas of Guangxi Zhuang Autonomous Region and confirmed to be subtype 4 a by HE sequencing. HEV RNA in blood, serum alanine aminotransferase (ALT), HEV antibody and liver tissue pathological and electron microscopic examination to determine HEV pathogenicity. Results The results showed that detoxification, viremia, elevated ALT and HEV antibody positive in swine HEV strains infected with rhesus monkeys occurred. The biopsy liver sections showed typical pathological changes of acute viral hepatitis. Electron microscopy showed that hepatocytes were scattered A large number of virus-like particles. Conclusion Guangxi 4-year-old pig HEV is capable of infecting macaques and causing hepatitis E infection across species.