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研究试验结果表明 :参试的四个甘蔗品种 ,茎尖脱毒培养效果从好到差的顺序依次为拔地拉 >F134 >粤糖 57/ 4 2 3>桂糖 11号。而同一品种不同的培养基、外源激素、培养方式和培养环境 ,茎尖培养效果差异也很大。在温室条件下 ,以 2 0 % S或改良 MS0 作预培养 3～ 5天后 ,再转入改良 MS+BA2 .0 mg/ L+NAA0 .1mg/ L培养基进行滤纸桥液体振动培养 ,并根据材料褐变情况及时转管 ,是茎尖培养的最佳途径
The results of the research showed that the best and worst performance of the four sugarcane cultivars from the top to the bottom were in the order of Pilatilla> F134> Yulose 57/4223> Guitang 11. The same variety of different media, exogenous hormones, culture methods and culture environment, shoot tip culture effect is also very different. Under greenhouse conditions, pre-culture with 20% S or modified MS0 for 3 to 5 days, and then transferred to the modified MS + BA2 .0 mg / L + NAA0.1mg / L medium for filter paper bridge liquid vibration culture, and according to Material browning timely transfer tube, is the best way to shoot culture