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石楠(Photinia serrulata Lindl.)是华中地区一种重要的绿化树种。近年来在武汉地区石楠上发现一种新的叶斑病。本文通过组织分离法、致病性测定等从病株分离得到致病病原菌,并通过形态学鉴定、分子生物学鉴定等,最终确定该致病菌为小孢拟盘多毛孢(Pestalotiopsis microspora)。在室内测定了P.microspora对7种常见杀菌剂的敏感性,结果表明,7种杀菌剂对P.microspora的菌丝生长均有抑制作用,但不同杀菌剂之间EC50值相差较大。450 g·L~(-1)咪鲜胺水剂、70%甲基托布津可湿性粉剂、10%苯醚甲环唑水分散粒剂EC50值分别为0.099、0.142和0.631μg·mL~(-1)。25%三唑酮可湿性粉剂EC50值在7种杀菌剂中最大,为37.625μg·mL~(-1)。这是国内外关于P.microspora引起石楠叶斑病的首次报道。
Photinia serrulata Lindl. Is an important greening species in Central China. In recent years, a new leaf spot has been found on heather in Wuhan. In this paper, pathogenic pathogens were isolated from diseased plants by tissue isolation and pathogenicity determination. The pathogenic bacteria were identified as Pestalotiopsis microspora by morphological identification and molecular biological identification. The sensitivity of P.microspora to seven common fungicides was determined indoors. The results showed that seven fungicides inhibited the mycelium growth of P.microspora, but the EC50 values differed greatly among the fungicides. EC50 of 450 g · L -1 prochloraz, 70% thiophanate-methyl and 10% difenoconazole were 0.099,0.142 and 0.631 μg · mL ~ (-1) -1). The EC50 value of 25% triadimefon WP was the highest among 37 fungicides, 37.625μg · mL -1. This is the first time that P.microspora caused leaf spot disease in southern China.