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加大化疗的强度联合造血干细胞移植可以挽救一批复发的HL、NHL、AML等病人的生命。HCT联合BMT/PBSCT已开始作为预后差、但化疗敏感肿瘤的首次治疗方案。为了缩短骨髓再生低下期、减少感染、减少移植中肿瘤细胞污染,利用体外筛选、培养的干细胞移植成为方向(Cooper DL 1994). 本文报告对21例难治性(常规化疗未完全缓解、复发的、转移)恶性肿瘤用活化的外周血干细胞移植的结果: PBSC_s用CS-3000 Plus分离机分离,循环血量:10000ml/每次,分离次数:2.8(2-4次),分得CD34+细胞累计量3.5×10~6/KG(1.9-11.2)。细胞活化:G/(GM-CSF、Anti-CD3 MoAb、IL-2,培养三天。工作程序:(0小时)GM-CSF→(24小时)CD3-MoAb/IL-2→(72小时)收集、移植。病人均接受了5～6倍常规剂量的化疗。
Increasing the intensity of chemotherapy combined with hematopoietic stem cell transplantation can save the lives of a group of patients with recurrent HL, NHL, and AML. HCT combined with BMT/PBSCT has begun as the first treatment option for poor prognosis but chemotherapy-sensitive tumors. In order to shorten the period of bone marrow regeneration, reduce infection, and reduce the contamination of tumor cells during transplantation, stem cell transplantation using in vitro screening and culture has become the direction (Cooper DL 1994). This article reports 21 cases of refractory (incomplete response to conventional chemotherapy, relapse And metastasis) Results of transplantation of activated peripheral blood stem cells for malignant tumors: PBSC_s was isolated with a CS-3000 Plus separator, circulating blood volume: 10000 ml/time, number of separations: 2.8 (2-4 times), and CD34+ cells were accumulated The amount of 3.5 × 10 ~ 6/KG (1.9-11.2). Cell activation: G/(GM-CSF, Anti-CD3 MoAb, IL-2, culture for three days. Working procedure: (0 hour) GM-CSF→ (24 hours) CD3-MoAb/IL-2→ (72 hours) The patients were all received 5 to 6 times the conventional dose of chemotherapy.