Derivatization Reaction of Carbohydrates with Urea as the Reagent and Fluorimetric Determination of

来源 :Chinese Journal of Chemistry | 被引量 : 0次 | 上传用户:sory520
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It is found that in the presence of sulfuric acid carbohydrates condense with urea to afford the condensation products, which emit fluorescence. Under optimum conditions, the fluorescence intensities of system are proportional to the concentrations of carbohydrates. Based on this linear relationship, quantitative determination of kinds of carbohydrates has been made. Among all the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits of detection reaches 3.5×10 -8 mol/L. So α-rhamnose can be selectively determined in the presence of other carbohydrates. A interaction mechanism is also discussed It is found that in the presence of sulfuric acid carbohydrates condense with urea to afford the condensation products which which emit fluorescence. Under optimum conditions, the fluorescence intensities of the systems are proportional to the concentrations of carbohydrates. Based on this linear relationship, quantitative determination of Among all the carbohydrates tested, the sensitivity of α-rhamnose is the highest and its limits of detection reaches 3.5 × 10 -8 mol / L. So α-rhamnose can be selected determined in the presence of other carbohydrates. A interaction mechanism is also discussed
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