用熔融-乳化法制备葫芦素B纳米脂质载体,以平均粒径、包封率和载药量为评价指标,采用星点设计-效应面法优化处方。所得优化处方为单硬脂酸甘油酯0.3 g、卵磷脂0.125 g、注射用大豆油0.075 g、聚乙二醇40硬脂酸酯0.2 g。优化处方平均粒径、包封率和载药量的实测值分别为120.8 nm、83.3%和0.83%,并与预测值相近。制品在45.5 h时体外释放率可达(87.1±3.5)%,且体外释放行为符合一级动力学模型。 Cucurbitacin B nano-lipid carrier was prepared by melt-emulsification method. The average particle size, entrapment efficiency and drug loading were used as evaluation indexes to optimize the formulation by using the star-point design-response surface method. The optimized formulation was 0.3 g of glyceryl monostearate, 0.125 g of lecithin, 0.075 g of soybean oil for injection, and 0.2 g of polyethylene glycol 40 stearate. The average particle size, encapsulation efficiency and drug loading of optimized prescription were 120.8 nm, 83.3% and 0.83%, respectively, which were close to the predicted value. In vitro release rate of the product reached (87.1 ± 3.5)% at 45.5 h, and the in vitro release behavior accorded with the first-order kinetic model.