Objective: To investigate the role of Hemocoagulase Injection used in the joint operation and the effect on the blood coagulable function. Methods: A total of 60 patients who undergoing joint operation in our hospital were randomly divided into two groups. Experiment group (Group A, n=30) was injected with 2 U Hemocoagulase Injection in 5 min before anesthesia induction. The contrast group (Group B, n=30) was treated with 0.9% normal saline in 5 min before anesthesia induction. Then common anesthesia was given to the two groups of patients. The venous blood was withdrawn for blood routine examination, thrombelastography and coagulable function at the time of pre-inducement, end of operation, and in 6 and 12 h after operation. The change of thrombelastograph and coagulable state were monitored during the whole surgery. And the volume of transfusion and hemorrhage between two groups was contrasted. Results: After the use of Hemocoagulase Injection, the operative time was shortened obviously (P＜0.05). The volumes hemorrhage and transfusion was obviously smaller in Group A than in Group B (P＜0.01). Hemocoagulase Injection did not influence fibrinogen and thrombelastograph of Group A (P＞0.05). Conclusion: Hemocoagulase Injection can reduce the volumes of hemorrhage and transfusion and not influence the coagulable function. It is worth using in the joint operation.