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目的 探讨全反式维甲酸（ Ａ Ｔ Ｒ Ａ） 、三氧化二砷（ Ａｓ２ Ｏ３） 及柔红霉素（ Ｄ Ｎ Ｒ） 对急性早幼粒细胞白血病（ Ａ Ｐ Ｌ） 细胞株 Ｎ Ｂ４ 细胞组织因子（ Ｔ Ｆ） 表达的影响。方法 用复钙时间检测 Ｎ Ｂ４ 细胞的促凝活性（ Ｐ Ｃ Ａ） ；用 Ｅ Ｌ Ｉ Ｓ Ａ 方法检测其 Ｔ Ｆ 抗原；用逆转录聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ） 检测 Ｔ Ｆｍ Ｒ Ｎ Ａ的转录水平。结果 Ａ Ｔ Ｒ Ａ 和 Ａｓ２ Ｏ３ 均呈时间依赖的方式下调 Ｎ Ｂ４ 细胞的 Ｐ Ｃ Ａ、 Ｔ Ｆ 抗原水平以及 Ｔ Ｆ ｍ Ｒ Ｎ Ａ的转录；而 Ｄ Ｎ Ｒ 处理 Ｎ Ｂ４ 细胞的 Ｐ Ｃ Ａ及 Ｔ Ｆ 抗原在早期上调，至２４ ～４８ 小时达到最大值。对 Ｐ Ｃ Ｒ 产物测序，发现 Ａ Ｐ Ｌ 细胞 Ｔ Ｆ 第５ 个外显子缺失的转录本。结论 Ａ Ｔ Ｒ Ａ 和 Ａｓ２ Ｏ３ 均可下调 Ａ Ｐ Ｌ 细胞 Ｔ Ｆ的表达并降低其 Ｐ Ｃ Ａ，改善 Ａ Ｐ Ｌ患者的弥散性血管内凝血（ Ｄ Ｉ Ｃ） 出血症状； Ａｓ２ Ｏ３ 和 Ｄ Ｎ Ｒ 对 Ａ Ｐ Ｌ凝血障碍不同效应的作用机制至少部分与药物对 Ａ Ｐ Ｌ细胞 Ｔ Ｆ 表达和 Ｐ Ｃ Ａ 的不同调节作用有关。
Objective To investigate the role of all-trans retinoic acid (A T R A), arsenic trioxide (As2 O3) and daunorubicin (D N R) in the acute tissue proliferative cell lineage (APL) cell line N B4 (T F) Influence of expression. Methods The time of recalcification was used to detect the procoagulant activity (P C A) of N B4 cells. The T F antigen was detected by E L I S A method. T was detected by reverse transcription polymerase chain reaction (RT-PCR). The transcription level of Fm R N A. Results Both A T R A and As2 O3 down-regulated the levels of P C A, T F antigen and transcription of T F m R N A in N B4 cells in a time-dependent manner, while D N R treated P C A in N B4 cells. The T F antigen was up-regulated early and reached its maximum value within 24 to 48 hours. Sequencing of the P C R product revealed that the A P L cell lost the transcript of the 5 th exon of the T F . Conclusion Both A T R A and As2 O3 can down-regulate the expression of FT and decrease P A of A P L cells, and improve the disseminated intravascular coagulation (D I C) bleeding in A P L patients; As 2 O 3 and D N The mechanism of action of R on the different effects of A P L coagulation disorders is at least partly related to the different regulatory effects of drugs on A F L cell T F expression and P C A .