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目的 探讨全反式维甲酸( A T R A) 、三氧化二砷( As2 O3) 及柔红霉素( D N R) 对急性早幼粒细胞白血病( A P L) 细胞株 N B4 细胞组织因子( T F) 表达的影响。方法 用复钙时间检测 N B4 细胞的促凝活性( P C A) ;用 E L I S A 方法检测其 T F 抗原;用逆转录聚合酶链反应( R T P C R) 检测 T Fm R N A的转录水平。结果 A T R A 和 As2 O3 均呈时间依赖的方式下调 N B4 细胞的 P C A、 T F 抗原水平以及 T F m R N A的转录;而 D N R 处理 N B4 细胞的 P C A及 T F 抗原在早期上调,至24 ~48 小时达到最大值。对 P C R 产物测序,发现 A P L 细胞 T F 第5 个外显子缺失的转录本。结论 A T R A 和 As2 O3 均可下调 A P L 细胞 T F的表达并降低其 P C A,改善 A P L患者的弥散性血管内凝血( D I C) 出血症状; As2 O3 和 D N R 对 A P L凝血障碍不同效应的作用机制至少部分与药物对 A P L细胞 T F 表达和 P C A 的不同调节作用有关。
Objective To investigate the role of all-trans retinoic acid (A T R A), arsenic trioxide (As2 O3) and daunorubicin (D N R) in the acute tissue proliferative cell lineage (APL) cell line N B4 (T F) Influence of expression. Methods The time of recalcification was used to detect the procoagulant activity (P C A) of N B4 cells. The T F antigen was detected by E L I S A method. T was detected by reverse transcription polymerase chain reaction (RT-PCR). The transcription level of Fm R N A. Results Both A T R A and As2 O3 down-regulated the levels of P C A, T F antigen and transcription of T F m R N A in N B4 cells in a time-dependent manner, while D N R treated P C A in N B4 cells. The T F antigen was up-regulated early and reached its maximum value within 24 to 48 hours. Sequencing of the P C R product revealed that the A P L cell lost the transcript of the 5 th exon of the T F . Conclusion Both A T R A and As2 O3 can down-regulate the expression of FT and decrease P A of A P L cells, and improve the disseminated intravascular coagulation (D I C) bleeding in A P L patients; As 2 O 3 and D N The mechanism of action of R on the different effects of A P L coagulation disorders is at least partly related to the different regulatory effects of drugs on A F L cell T F expression and P C A .