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目的:研究二十二碳六烯酸(DHA)、二十碳五烯酸(EPA)对模拟失重大鼠红细胞膜流动性的影响。方法:将30只健康SD大鼠随机分为地面对照组(Ⅰ)、悬吊对照组(Ⅱ)和悬吊实验组(Ⅲ)。悬吊实验组鼠料中添加DHA、EPA,其他2组为普通鼠料。悬挂40d后利用激光衍射法测定红细胞变形指数、取向指数,荧光偏振法测定红细胞膜流动性,硫代巴比妥酸荧光法测定红细胞膜脂质过氧化物(LP0),激光共聚焦显微镜观测红细胞纤维型肌动蛋白的改变及含量。结果:失重下大鼠红细胞的变形指数、取向指数以及红细胞膜的流动性明显降低(P<0.05),LPO增加,红细胞形态和骨架发生改变,F-actin含量明显降低(P<0.05)。结论:在模拟失重条件下,红细胞膜流动性降低,DHA、EPA可拮抗损伤作用,对红细胞膜具有较强的保护作用。
Objective: To study the effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on the fluidity of erythrocyte membrane in simulated weightless rats. Methods: Thirty healthy SD rats were randomly divided into ground control group (Ⅰ), suspension control group (Ⅱ) and suspension test group (Ⅲ). DHA and EPA were added to the suspension experimental group rats, while the other two groups were ordinary rat materials. After 40 days of suspension, the erythrocyte deformation index and orientation index were measured by laser diffraction method, the erythrocyte membrane fluidity was measured by fluorescence polarization method, the erythrocyte membrane lipid peroxide (LP0) was measured by thiobarbituric acid fluorescence method, Changes and content of fibrillar actin. Results: The deformation index, orientation index and erythrocyte membrane fluidity of rat erythrocyte under weightlessness decreased significantly (P <0.05). LPO increased, erythrocyte shape and skeleton changed, F-actin content decreased significantly. CONCLUSION: Under the condition of simulated weightlessness, the fluidity of erythrocyte membrane is reduced. DHA and EPA can antagonize the injury and have a strong protective effect on erythrocyte membrane.