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目的探讨成人成肌细胞能否转分化为神经前体细胞。方法从6例成人正常颞肌标本中分离培养成肌细胞,培养至第3代时加入含有碱性成纤维细胞生长因子、表皮生长因子和白血病抑制因子的无血清培养液,诱导2周,对诱导细胞进行免疫细胞化学鉴定和逆转录聚合酶链反应(RT-PCR)分析;将诱导细胞和人成肌细胞分别移植到12只脑缺血大鼠脑内,采用免疫组织化学植细胞的存活和分化进行鉴定。结果成肌细胞在经诱导7 d 后开始聚集成球,悬浮生长,该细胞球平均每14 d 传代1次。细胞球均表达巢蛋白,在分化条件下,(36±6)%的细胞表达微管相关蛋白2,(44±5)%的细胞表达胶质纤维酸性蛋白,(17±4)%的细胞表达半乳糖脑苷脂;经诱导细胞移植到大鼠脑内后表达微管相关蛋白2和胶质纤维酸性蛋白。而对照组的成肌细胞则不表达上述标志物。结论人成肌细胞在体外一定诱导条件下能够转分化为神经前体细胞。
Objective To investigate whether adult myoblasts can differentiate into neural precursors. Methods Myoblasts were isolated and cultured from 6 adult normal temporomandibular muscle specimens. After passage 3, serum-free medium containing basic fibroblast growth factor, epidermal growth factor and leukemia inhibitory factor was added and induced for 2 weeks. Induced cells were identified by immunocytochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). The induced cells and human myoblasts were transplanted into the brains of 12 rats with cerebral ischemia respectively. The survival of the cells was detected by immunohistochemistry And differentiation were identified. Results Myoblasts began to accumulate into spheres after 7 days of induction and grew in suspension. The spheres were passaged once every 14 days. Nestin was expressed in the cytoplasm. Under differentiation, (36 ± 6)% of the cells expressed microtubule-associated protein 2, (44 ± 5)% of the cells expressed glial fibrillary acidic protein, (17 ± 4)% of the cells Expression of galactocerebroside; expression of microtubule-associated protein 2 and glial fibrillary acidic protein after transplanted into rat brain. The control group of myoblasts did not express the above markers. Conclusion Human myoblasts can differentiate into neural progenitor cells under certain inducing conditions in vitro.