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以地被菊‘矮黄’、‘玉龙’和传统菊花‘金背大红’的叶盘和茎段为外植体进行再生培养 .实验结果表明 ,不同品种对相同激素水平的反应是不同的 :传统的大菊‘金背大红’的愈伤诱导率和分化率都远远低于两种地被菊 ;在相同条件下以茎段为外植体诱导愈伤率高于叶盘 ,但是叶盘的直接分化率高于茎段 .‘玉龙’和‘矮黄’的最适再生培养基组成为MS +NAA 0 .1mg/L + 6 BA 1mg/L和MS +NAA 0 .2mg/L + 6 BA 2mg/L ,‘金背大红’为MS +NAA 1mg/L + 6 BA 5mg/L
The leaves and stems of leaves, leaves and stems of daisy yellow, jade dragon and red chrysanthemum were used as explants for regeneration.The results showed that the responses of different varieties to the same hormone level were different: The callus induction rate and differentiation rate of the traditional big-daisy ’durianhong’ were much lower than those of the two kinds of damaging chrysanthemums. Under the same conditions, the induction rate of callus by the stem segment was higher than that of the leaf disc, The direct regeneration rate of ’Yulong’ and ’short yellow’ was MS + NAA 0 .1mg / L + 6 BA 1mg / L and MS + NAA 0.2mg / L + 6 BA 2mg / L, ’dorsal red’ is MS + NAA 1mg / L + 6 BA 5mg / L