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目的探讨血管生成素1(angi.Poietin-1,Ang-1)对糖尿病大鼠股骨头微血管新生及渗漏的影响。方法建立速发型链脲佐菌素(streptozotocin,STZ)糖尿病大鼠模型,随机分为正常5周组(CON1)、10周组(CON2)及15周组(CON3),糖尿病5周组(DM1)、10周组(DM2)及15周组(DM3),每组10只。墨汁灌注观测股骨头微血管密度;摘取模型动物股骨头组织,免疫组化分析凝血因子Ⅷ(FⅧ)表达;原位杂交分析血管内皮生长因子(VEGFmRNA)表达强度;RT-PCR分析Ang-1的mRNA表达。结果糖尿病大鼠股骨头随病程发展,Ang-1、FⅧ因子表达上升,与正常组相比有显著性差异(P<0.01)。VEGFmRNA表达量均高于正常组(P<0.01);微血管密度加大,显示血管增生、渗漏。结论糖尿病股骨头Ang-1与VEGFmRNA相互协同或拮抗分别促进微血管增生、抗血管渗漏。表达于血管内皮细胞的FⅧ及VEGFmRNA与微血管密度(MVD)变化存在正相关。
Objective To investigate the effects of Angi-Potin-1 (Ang-1) on neovascularization and leakage of femoral head capillaries in diabetic rats. Methods A rat model of rapid onset streptozotocin (STZ) was established and randomly divided into 5 groups: CON1, CON2, CON3, DM1 ), 10 weeks group (DM2) and 15 weeks group (DM3), 10 rats in each group. Ink perfusion was used to observe the microvessel density of the femoral head. The femoral head tissue of the animal model was removed, the expression of coagulation factor Ⅷ (FⅧ) was analyzed by immunohistochemistry, the expression of vascular endothelial growth factor (VEGF) mRNA expression. Results The expression of Ang-1 and FⅧ in the femoral head of diabetic rats increased with the progression of the disease, which was significantly different from the normal group (P <0.01). VEGF mRNA expression levels were higher than the normal group (P <0.01); microvessel density increased, showing vascular proliferation and leakage. Conclusions Angiotensin-1 and VEGF mRNA in the femoral head of diabetic rats synergize with each other or antagonize the proliferation of microvascular and anti-vascular leakage respectively. There was a positive correlation between FⅧ and VEGF mRNA expression in vascular endothelial cells and microvessel density (MVD).