论文部分内容阅读
以8个银耳菌株为材料,研究交配型因子A在不同菌株中的分布,菌株内及菌株间单孢分离 物配对试验结果表明,此8个菌株中只有两个不同的A因子,与前人报道的银耳是限制型双因子交配系 统的报道是一致的。研究了培养条件对银耳孢子产交配信息素的影响,结果表明银耳孢子产交配信息素 的较佳培养基为:蔗糖20 g/L,(NH4)2SO4 1.584 g/L(含N 24 mmol/L),MgSO4·7H2O 0.25 g/L, KH2PO4·3H2 O 0.5 g/L,VB1 0.2 mg/L,ZnSO4·7H2O 2 mg/L,CaCl2·2H2O 0.5 g/L,钼酸铵0. 02 mg/L。最佳发酵条件为培养基pH 6.5,23℃,140 r/min,在优化培养基和培养条件下,培养4 d发酵 液中交配信息素的活性最高,可达到12 U/mL,是在基础培养基中活力的5～6倍。银耳交配信息素经胃蛋白酶处理后,活性完全丧失,由此推测,银耳交配信息素是一种多肽类物质。
Eight strains of fungus were used to study the distribution of mating factor A in different strains. The results of pairing test of single spore isolates in and between strains showed that there were only two different A factors in these eight strains, Reported Tremella is a confined two-factor mating system reported is consistent. The effects of culture conditions on the pheromone production of Tremella fuciformis were studied. The results showed that the optimum culture medium for the production of pheromone was trehalose 20 g / L, (NH4) 2SO4 1.584 g / L (containing N 24 mmol / L ), MgSO4 · 7H2O 0.25 g / L, KH2PO4 · 3H2 O 0.5 g / L, VB1 0.2 mg / L, ZnSO4 · 7H2O 2 mg / L, CaCl2 · 2H2O 0.5 g / . The optimal fermentation conditions were pH 6.5, 23 ℃ and 140 r / min. Under optimized medium and culture conditions, the highest activity of mating pheromone was reached at 4 days in fermentation broth, which reached 12 U / mL, 5 to 6 times the vitality of the medium. Tremella-mating pheromone treated by pepsin, the activity was completely lost, presumably, Tremella mate pheromone is a polypeptide material.