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目的探讨几项细胞块免疫化学染色鉴别浆膜腔积液中增生的间皮细胞和腺癌细胞。方法收集腺癌、间皮增生的浆膜腔积液66例,制成细胞块切片,进行HE及癌胚抗原(CEA)、钙视膜蛋白(Calretinin)、波形蛋白(vimen-tin)免疫化学染色。结果普通涂片发现浆膜腔积液中转移性腺癌46例,间皮细胞增生20例,CEA在46例转移性腺癌中,32例阳性,14例阴性,阳性率69.57%;20例增生的间皮细胞中2例阳性(均为弱阳性),18例阴性,阳性率10.00%。46例转移性腺癌中calretinin 1例阳性,45例阴性,阳性率2.17%;20例增生的间皮细胞17例阳性,3例阴性,阳性率85.00%。46例转移性腺癌中vimentin阳性表达2例,44例阴性,阳性率4.35%;20例增生的间皮细胞中18例阳性,2例阴性,阳性率90.00%,差异有统计学意义(P<0.01)。结论细胞块免疫化学染色对鉴别浆膜腔积液中增生的间皮细胞及腺癌细胞有重要参考价值。
Objective To investigate the immunocytochemical staining of several cell masses to identify mesothelial cells and adenocarcinoma cells in hyperplastic serous effusions. Methods Sixty-six patients with adenocarcinoma and mesodermal hyperplasia were collected and made into sections of cells for HE staining, CEA, Calretinin, vimen-tin immunochemistry dyeing. Results In the common smear, 46 cases of metastatic adenocarcinoma in serous effusions and 20 cases of mesothelial cell hyperplasia were found. Among 46 cases of metastatic adenocarcinoma, 32 cases were positive for CEA, 14 cases were negative and the positive rate was 69.57% Mesothelium in 2 cases were positive (all weak positive), 18 negative, the positive rate of 10.00%. One case of metastatic adenocarcinoma was positive for calretinin, 45 cases were negative and the positive rate was 2.17%. Twenty cases of hyperplastic mesothelial cells were positive, 3 cases were negative, and the positive rate was 85.00%. In 46 cases of metastatic adenocarcinoma, vimentin positive expression was found in 2 cases, 44 cases were negative and the positive rate was 4.35%. 18 cases were positive in 20 cases of hyperplastic mesothelioma, 2 cases were negative and the positive rate was 90.00%, the difference was statistically significant (P < 0.01). Conclusion Immunocytochemical staining of the cell mass is an important reference for differentiating mesodermal and adenocarcinoma cells from serosal effusion.