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目的 研究饮用水中2,6-二氯-1,4-苯醌(2,6-DCBQ)对小鼠的急性毒性和遗传毒性作用.方法 选择健康6～8周龄SPF级昆明小鼠进行试验.将50只小鼠随机分为5组,分别为阴性对照(玉米油)组和215、464、1 000、2 150 mg/kg2,6-DCBQ染毒组,每组10只,雌雄各半.采用一次性经口灌胃方式进行染毒,染毒容量为20 ml/kg.染毒后观察14d,记录动物死亡数,计算其半数致死剂量(median lethal dose,LD50).将50只小鼠随机分为5组,分别为溶剂对照(玉米油)组和62.5、125和250 mg/kg 2,6-DCBQ染毒组及阳性对照组(40 mg/kg环磷酰胺),每组10只,雌雄各半.采用灌胃方式进行染毒,染毒容量为20 ml/kg,两次灌胃间隔24h;第二次灌胃后6h,进行骨髓细胞微核试验.将50只雄性小鼠随机分为5组,分别为溶剂对照(玉米油)组和62.5、125和250 mg/kg 2,6-DCBQ染毒组及阳性对照组(40 mg/kg环磷酰胺),每组10只.采用经口灌胃方式进行染毒,染毒容量为20 ml/kg,连续灌胃5d.染毒后第35天,进行精子畸形试验.结果 2,6-DCBQ对雄性、雌性小鼠经口急性毒性的LD50分别为501 mg/kg(95％CI:344～730 mg/kg)和584 mg/kg(95％CI:430～794mg/kg).不同剂量2,6-DCBQ染毒组小鼠的骨髓细胞微核率及精子畸形率与溶剂对照组比较,差异均无统计学意义(P＞0.05).各组PCE/NCE值均在正常范围内.结论 2,6-DCBQ对小鼠经口急性毒性属于低毒级,且未发现其具有遗传毒性.“,”Objective To evaluate acute toxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone (2,6-DCBQ) in drinking water in mice.Methods For LD50 calculation,a total of 50 SPF grade Kunming (KM) mice aged 6-8 months were randomly divided into five groups,10 in each group(male∶female=1∶1),the negative control (corn oil) group and four exposure groups(215,464,1 000 and 2 150 mg/kg body weight).2,6-DCBQ were administered by garage,20 ml/kg once.A total of 50 Kunming mice were randomly divided into five groups,10 in each group (male∶female=1∶1).The test groups were treated with 2,6-DCBQ through gavage at the doses of 62.5,125,and 250 mg/kg body weight respectively.The positive control group was treated by cyclophosphamide (40 mg/kg body weight).The negative control group was treated by corn oil.The mice were treated by gavage with 20 ml/kg for twice at 24 h intervals.Bone marrow cell micronucleus test was conducted after the second treatment at 6 h intervals.Sperm abnormality test was conducted with 50 male Kunming mice.They were randomly divided into five groups,10 in each group.The test groups were treated with 2,6-DCBQ through gavage at the doses of 62.5,125,and 250 mg/kg body weight respectively.The positive control was cyclophosphamide (40 mg/kg body weight).The negative control was corn oil.The mice were treated by gavage with 20 ml/kg for five consecutive days.The rate of sperm abnormality of mice induced by 2,6-DCBQ were observed at the 35th days after exposure.Results LD50 and 95％CI of male and female mice were 501 mg/kg body weight(95％CI:344-730 mg/kg body weight) and 584 mg/kg body weight (95％CI:430-794 mg/kg body weight) respectively in acute oral toxicity test.After exposure to 2,6-DCBQ,differences in micronucleus frequency of bone marrow cells and rate of sperm abnormality between exposure groups and solvent control group had no statistical significance (P＞0.05).The ratios of PCE/NCE in each group were in the normal reference range.Conclusion In acute oral toxicity test,2,6-DCBQ is identified as the lower toxic chemicals.It does not increase micronucleus frequency and the rate of sperm abnormality in mice.