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目的建立祛白酊的质量控制方法。方法采用微乳薄层色谱法,对祛自酊中的枙子、红花进行定性鉴别;以Diamonsil C_(18)(250 mm×4.6 mm,5μm)为固定相,采用HPLC法对栀子中有效成分栀子苷和红花中有效成分羟基红花黄色素A进行含量测定,以(A)乙腈-水(15:85),(B)甲醇-0.83%磷酸溶液(30:70)为流动相,梯度洗脱,检测波长:0~10 min,238nm,10~20 min,403 nm,流速:1.0 ml·min~(-1),柱温:25℃,进样量:10μl。结果栀子和红花薄层色谱中与对照药材主斑点相应位置斑点清晰,阴性对照无干扰;栀子苷和羟基红花黄色素A分别在9.6~144.0、2.4~36.0μg·ml~(-1)范围内线性关系良好,加样回收率分别为100.30%和100.08%。结论本方法简便、准确、重复性好,可作为祛白酊的质量控制方法。
Objective To establish a quality control method of Qu Bai tincture. Methods Diamonsil C_ (18) (250 mm × 4.6 mm, 5 μm) was used as the stationary phase and the HPLC method was used to determine the content of Rhizoma (A) Acetonitrile-water (15:85), (B) Methanol-0.83% phosphoric acid solution (30:70) for the determination of the active ingredient Geniposide and Safflower yellow Phase and gradient. The detection wavelength was 0-10 min, 238 nm, 10-20 min, 403 nm, and the flow rate was 1.0 ml · min -1. The column temperature was 25 ° C and the injection volume was 10 μl. Results The results showed that the spots of Zhizi and Safflower TLC on the spots corresponding to the main spot of control were clear and no interference was found on the negative control. Geniposide and hydroxysafflor yellow A were detected at 9.6 ~ 144.0,2.4 ~ 36.0μg · ml ~ (- 1) within a good linear relationship, sample recovery rates were 100.30% and 100.08%. Conclusion The method is simple, accurate, reproducible, and can be used as a quality control method of Qu Bai tincture.