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本文研究了水溶性试剂１－（２－吡啶偶氮）－２蔡酚－６－磺酸（ＰＡＮ－Ｓ）与Ｆｅ￣２＋、ＣＯ￣＋、Ｎｉ￣２＋之鳌合物的衍生和液相色谱分离条件，在Ｃ＿１８柱上，用含１０ｍｍｏｌ／Ｌ的ｐＨ５．０的ＨＡｃ－ＮａＡｃ缓冲溶液的甲醇水溶液（５０：５０，ｖ／ｖ）作流动相，溴化四丁基胺（ＴＢＡ－Ｂｒ）作对离子试剂，流动相流速为１．０ｍｌ／ｍｉｎ，在５５０ｎｍ波长处进行光度检测。在１１ｍｉｎ内分离测定了Ｆｅ￣（２＋）、Ｃｏ￣（２＋）、Ｎｉ￣（２＋）与ＰＡＮ－Ｓ的鳌合物，提出了离子对反相高效液相色谱快速分离测定痕量铁、钴、镍的新方法。信噪比Ｓ／Ｎ＝２时，铁、钻、镍的检测限分别为０．０４３、０．００７、０．０１２。方法用于同时测定试剂和茶叶中铁、钴、镍的含量，与原子吸收分光光度法测定结果一致。
In this paper, the derivatization and liquid phase of the chelate of water-soluble reagent 1- (2-pyridylazo) -2-phenyl-6-sulfonic acid (PAN-S) with Fe 2+, CO 2+, Ni 2+ Chromatography was performed on a C 18 column using 10 mmol / L HAc-NaAc buffer solution in methanol (50:50, v / v) at pH 5.0 as the mobile phase and tetrabutylammonium bromide (TBA-Br ) As a counterion, the mobile phase flow rate of 1.0ml / min, at a wavelength of 550nm photometric detection. The chelates of Fe ~ (2 +), Co ~ (2+), Ni ~ (2+) and PAN-S were separated and determined within 11 min. The ion-pair reversed-phase high performance liquid chromatography , A new method of nickel. S / N = 2, the detection limits of iron, drill, nickel were 0.043,0.007,0.012. The method was applied to the simultaneous determination of iron, cobalt, and nickel in reagents and tea, consistent with the results of atomic absorption spectrometry.