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目的了解长牡蛎(Crassostrea gigas)消化道组织中类组织血型抗原(histo-blood group antigens,HBGAs)的类型特点,分析其与诺如病毒的结合特性,以探讨长牡蛎富集诺如病毒的机制。方法利用8种HBGAs单克隆抗体,建立长牡蛎中类HBGAs检测的ELISA方法,并分析其主要型别。同时,利用5种体外表达的GII.4型诺如病毒P粒子分析其与长牡蛎中类HBGA的结合特性。结果长牡蛎消化道组织中存在类A,H1,Lea和Ley型HBGA;55019株(2006b变异株)和97-1l株(95/96US变异株)GII.4型诺如病毒P粒子可通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合,91(Camberwell_91株)和42(Hunter_2004)株可通过类Ley型HBGA与长牡蛎消化道组织相结合,156株(sakai株)不与任何类型类HBGA结合。结论长牡蛎消化道组织中存在类A、H1、Lea和Ley型HBGA,GII.4型诺如病毒主要通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合。
OBJECTIVE: To investigate the types and characteristics of histo-blood group antigens (HBGAs) in the digestive tract of Crassostrea gigas and analyze its binding characteristics with Norovirus so as to explore the mechanism of enrichment of Norovirus . Methods Eight kinds of HBGAs monoclonal antibodies were used to establish the ELISA method for detection of HBsAg in long-oyster. The main types of HBsAg were detected. In the meantime, the binding characteristics of five Norovirus GII-type GII.4 particles in vitro to HBGA in long-oyster were analyzed. Results The genotypes A, H1, Lea and Ley HBGA were found in digestive tract of Oyster oyster. 55019 strains (2006b mutant) and 97-1l strains (95/96 strain) A, H1, and Ley-type HBGA were combined with gypenosponsical tissues of Oyster oyster. 91 (Camberwell_91) and 42 (Hunter_2004) strains could be combined with digestive tract tissues of Oyster oysters via Ley-like HBGA. Any type of HBGA combined. Conclusion The genotypes A, H1, Lea and Ley HBGA are present in the digestive tract of Long Oyster. The norovirus of GII.4 mainly binds to the digestive tract tissue of Oyster Oyster through the class A, H1 and Ley HBGA.