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目的:观察心康冲剂对慢性心衰大鼠AVP m RNA表达的影响。方法:70只SD大鼠随机抽取10只为正常对照组,剩余大鼠腹腔注射盐酸多柔比星建立CHF模型,再随机分为模型对照组、心康冲剂低中高剂量组、芪苈强心组(对照组),每组各12只。给药治疗第6周,各组随机取存活大鼠6只,开始收集大鼠24 h尿液(1次/d,连续7 d),实验结束后检测大鼠下丘脑AVP m RNA的表达情况。结果:与正常对照组比较,模型组的AVP m RNA表达显著升高,24 h尿量明显减少,差异有统计学意义(P<0.05),表明造模成功;与模型组比较,芪苈强心组、心康冲剂各组AVP m RNA表达显著下降,各组尿量均显著增加(P<0.05);与芪苈强心组比较,心康冲剂各组的AVP m RNA表达差异显著,各组尿量均显著增加(P<0.05);心康冲剂各组间比较AVP m RNA表达及尿量差异无统计学意义(P>0.05)。结论:心康冲剂通过下调心衰大鼠AVP m RNA表达,抑制下丘脑释放AVP从而减少水重吸收,增加尿量,可能系其对慢性心衰利尿消肿的作用机制之一。
Objective: To observe the effect of xinkang granules on AVP mRNA expression in chronic heart failure rats. Methods: Seventy SD rats were randomly selected as the normal control group. The remaining rats were given intraperitoneal injection of doxorubicin hydrochloride to establish the CHF model, and then randomly divided into model control group, low and medium dose Xinxinkeli group, Group (control group), 12 in each group. At the 6th week of treatment, 6 rats in each group were randomized to receive 24 h urine (once / d for 7 days). After the experiment, the expression of AVP m RNA in the hypothalamus of rats was detected . Results: Compared with the normal control group, the expression of AVP m RNA in the model group was significantly increased and the urine output in 24 h was significantly decreased (P <0.05), indicating that the modeling was successful. Compared with the model group, The expression of AVP m RNA in heart and heart-Kang granules group decreased significantly, while the urine volume in each group increased significantly (P <0.05). Compared with Qiliqiangxin group, the expression of AVP m RNA in each group was significantly different (P <0.05). There was no significant difference in the expression of AVP m RNA between different groups and the urine volume (P> 0.05). Conclusion: Xinkang Granule can reduce AVP m RNA expression in heart failure rats, and inhibit AVP release from the hypothalamus to reduce water reabsorption and increase urine output, which may be one of the mechanisms of Xinkang granule on diuretic and detumescence of chronic heart failure.