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目的 探讨自由基致衰老的小鼠模型 DNA氧化损伤的情况。方法 通过给 2月龄小鼠吸入一定量的 O3,建立 O3衰老小鼠模型 ;然后以单细胞凝胶电泳试验检测 O3衰老小鼠模型、自然衰老小鼠和正常青年小鼠 (阴性对照组 )的脾淋巴细胞 DNA氧化损伤情况。结果 与阴性对照组相比 ,O3衰老小鼠模型的脾淋巴细胞DNA受到较显著的氧化损伤 (P<0 .0 5 ) ;其损伤程度与自然衰老小鼠相近 (P>0 .0 5 )。结论 O3衰老动物模型与自然衰老近似 ,在衰老的研究和抗衰老药物的药理实验中具有一定应用价值。
Objective To investigate the oxidative DNA damage induced by free radicals in a mouse model of aging. Methods O3 aging mice model was established by inhaling a certain amount of O3 into 2-month-old mice. Then the model of O3 aging mice, natural aging mice and normal young mice (negative control group) were detected by single cell gel electrophoresis. Splenic lymphocyte DNA oxidative damage. Results Compared with the negative control group, DNA of spleen lymphocytes of O3-aged mice was significantly oxidatively damaged (P <0.05), and the damage was similar to that of natural aging mice (P> 0.05) . Conclusion O3 aging animal model and natural aging similar, in the aging of the pharmacological experiments and anti-aging drugs has a certain value.