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目的：探讨血管紧张素转换酶抑制剂卡托普利、钙离子拮抗剂地尔硫 和β－激动剂多巴酚丁胺对大鼠主动脉血管内皮细胞单层通透性的影响。方法：大鼠主动脉内皮细胞（ＡＥＣ）培养采用陈思锋的无创性分离法分离培养；将ＡＥＣ培养在混合纤维素酯微孔滤膜上，培养８ｄ后进行通透性测定。长有ＡＥＣ的滤膜分别用卡托普利、地尔硫 和多巴酚丁胺处理４ｈ，置于灌注装置上，用含异硫氰酸荧光素标记白蛋白的高脂血灌注，测定收集液的量和白蛋白浓度，同时采用酶联法测定滤出液的总胆固醇（Ｔｃｈ）、甘油三酯（ＴＧ）、载脂蛋白Ａ和Ｂ（ａＰＡ、ａＰＢ）浓度。结果：三种药物均可降低ＡＥＣ对水、Ｔｃｈ、ＴＧ、ａＰＡ和ａＰＢ的通透性，其中多巴酚丁胺作用最为显著。而对白蛋白清除率除地尔硫 作用较显著外，其他两药均无明显作用。结论：卡托普利、地尔硫 和多巴酚丁胺可减少脂类和载脂蛋白渗入血管内皮下，从而预防和改善动脉粥样硬化。
Objective: To investigate the effects of angiotensin converting enzyme inhibitor captopril, calcium ion antagonist diltiazem and β-agonist dobutamine on monolayer permeability of aortic vascular endothelial cells in rats. Methods: AEC cultured in vitro was isolated and cultured by Chen Sifeng noninvasive separation method. AEC was cultured in a mixed cellulose ester microporous membrane filter and cultured for 8 days. AEC filters were treated with captopril, diltiazem and dobutamine for 4h, placed in a perfusion device, with fluorescein isothiocyanate-labeled albumin in hyperlipemia perfusion, the determination of the collection Liquid volume and albumin concentration, and the concentration of total cholesterol (Tch), triglyceride (TG), apolipoprotein A and B (aPA, aPB) in the filtrate was determined by enzyme-linked reaction. Results: All three drugs could decrease the permeability of AEC to water, Tch, TG, aPA and aPB, and the effect of dobutamine was the most significant. The albumin clearance except diltiazem more significant effect, the other two drugs had no significant effect. Conclusion: Captopril, diltiazem and dobutamine reduce the penetration of lipids and apolipoproteins into the vascular endothelium, thereby preventing and improving atherosclerosis.