本方法采用高压排斥色谱法分析香菇多糖。选用ＴＳＫＳＷ凝胶排斥色谱柱为分离柱，香菇样品经简单的预处理，在示差折光检测器中进行检测，以不同分子量的标准右旋糖酐（Ｄｅｘｔｒａｎ）作标准，同时测定样品中多糖的分子量分布情况及其含量。该方法较其他多糖测定法具有快速、简便、准确等优点，是目前较为行之有效的测定方法。 The method uses high pressure exclusion chromatography to analyze lentinan. The column of TSKSW gel exclusion column was used as the separation column. The mushroom samples were pretreated with simple refractive index detector, and the standard dextran (Dextran) of different molecular weight was used as the standard. Meanwhile, the molecular weight distribution of the polysaccharide in the sample was determined. Its content. This method is more rapid, simple and accurate than other polysaccharide assay, which is a more effective method.