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目的观察大鼠二次脑损伤合并海水浸泡后脑组织病理改变。方法利用改良二次脑损伤动物模型,对创伤处进行持续海水浸泡,分别在1、3、6、12、48h对挫伤侧海马区取材,固定于10%福尔马林液中,作HE染色,光镜下观察脑组织病理改变。结果二次脑损伤合并海水浸泡组出现脑水肿早于单纯二次脑损伤组,HE染色病理学改变观察表明海水浸泡能在早期加重脑水肿,后期损伤更严重,呈网格状改变,单纯二次脑损伤组脑组织呈海绵状改变。结论对应时间窗下,SBI合并海水浸泡比单纯SBI严重,SBI合并海水浸泡伤程度发展更迅速。
Objective To observe the pathological changes of brain tissue after secondary brain injury and seawater immersion in rats. Methods The animal model of the improved secondary brain injury was used, and the wounds were immersed in seawater. The injured hippocampus was harvested at 1, 3, 6, 12 and 48 hours respectively and fixed in 10% formalin solution for HE staining The pathological changes of brain tissue were observed under light microscope. Results Brain edema in the second brain injury combined with seawater immersion group was earlier than that in the second brain injury group. The pathological changes of HE staining showed that seawater immersion could aggravate cerebral edema in the early stage, The brain tissue in sub-brain injury group showed spongeous changes. Conclusions Under the corresponding time window, SBI combined with seawater immersion is more serious than pure SBI, SBI combined with seawater immersion injury developed more rapidly.