以芦丁标准品为对照,利用反相高效液相色谱法对芦笋中黄酮类化合物芦丁的含量进行定量测定。采用Agilent Eclipse XDB-C18色谱柱,柱温25℃,流动相由甲醇-水-磷酸(55∶44.5∶0.5)组成,流速为0.7 mL/min,检测波长390 nm。结果表明黄酮类化合物中各组分基线分离良好;进样量在0.07~0.7μg/20μL范围内,峰面积A与进样浓度C呈良好的线性关系,回归方程为A=1 5176C-10.388,相关系数R2=0.999 4;加样回收率为101.051%,RSD=3.306%;以保留时间和峰面积作精密度试验,RSD分别为0.199%和1.24%。该方法样品处理简单,准确度高,精密度好,适合于芦笋中芦丁含量的测定。 Using rutin standard as control, the content of rutin in asparagus in asparagus was quantitatively determined by reversed-phase high performance liquid chromatography. The Agilent Eclipse XDB-C18 column was used. The mobile phase consisted of methanol-water-phosphoric acid (55:44.5:0.5) at a flow rate of 0.7 mL / min at a detection wavelength of 390 nm. The results showed that the baseline of flavonoids was well separated. The injection volume was in the range of 0.07 ~ 0.7μg / 20μL, the peak area A was linear with the injection concentration C, the regression equation was A = 15176C-10.388, The correlation coefficient R2 = 0.999 4; the recovery rate was 101.051% and the RSD was 3.306%. The RSD was 0.199% and 1.24% with the retention time and peak area respectively. The method has the advantages of simple sample processing, high accuracy and good precision, and is suitable for the determination of rutin in asparagus.