论文部分内容阅读
本文用快速液相色谱（FPLC）从G2m（23）阳性人血浆中纯化出IgG2蛋白，免疫BALB/c小鼠，建立了一株分泌抗人G2m（23）单克隆抗体的细胞株，定名为2G8。经抑制ELISA，双抗体ELISA及斑点ELISA分析，证明2G8抗体具有G2m（23）单一特异性。
In this paper, IgG2 protein was purified from G2m (23) positive human plasma by fast liquid chromatography (FPLC) and immunized BALB / c mice to establish a cell line secreting anti-human G2m (23) monoclonal antibody 2G8. The inhibitory ELISA, double antibody ELISA and dot ELISA analysis demonstrated that the 2G8 antibody has a single specificity of G2m (23).