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目的探索miR200c稳定过表达对寡灶型转移肿瘤细胞株体外癌症生物学特性的影响。方法通过慢病毒感染建立稳定上调miR200c表达的寡灶型转移细胞株MDA-435-OL-miR200c(实验组),并同时建立阴性对照组(感染空质粒LV3NC)和阳性对照组(多灶型细胞株MDA-435-POL),通过CCK-8增殖曲线测定、琼脂平板克隆实验、Transwell小室迁移和侵袭实验来阐明上调miR200c对寡灶型转移细胞体外特性的影响以及上调目的基因后寡灶型与多灶型转移细胞株体外癌症生物学特性的不同和联系。结果在稳定寡灶转移细胞株中过表达miR200c对细胞体外克隆形成率为(0.69±0.02),迁移细胞数为(21.20±2.35),侵袭细胞数为(56.80±5.22),均较阴性对照组显著增加(P<0.05),与阳性对照组体外特性基本一致。但miR200c过表达对增殖能力没有显著的影响。结论寡灶型细胞株稳定上调miR200c后体外克隆形成能力、迁移能力和侵袭能力均有所提高,且呈现出多灶型细胞株的特性。
Objective To investigate the effect of stable miR200c overexpression on the biological characteristics of cancer in vitro in tumor-bearing tumor cells. Methods The lentiviral vector was used to establish the overexpression cell line MDA-435-OL-miR200c (experimental group) which up-regulated miR200c expression. At the same time, a negative control group (empty plasmid LV3NC) and a positive control group Strain MDA-435-POL). The effects of up-regulation of miR200c on the in vitro characteristics of the oligodendrocyte-transferred cells were investigated by CCK-8 proliferation assay, agar plate clone assay, Transwell chamber migration and invasion assay. Differences and relations between biological characteristics of multifocal metastatic cancer cell lines in vitro and in vitro. Results The rate of clonal formation in vitro was (0.69 ± 0.02), the number of migrating cells was (21.20 ± 2.35) and the number of invasive cells was (56.80 ± 5.22), respectively, compared with the negative control group (P <0.05), which was consistent with the in vitro characteristics of the positive control group. However, miR200c overexpression had no significant effect on proliferation. Conclusion The oligocarrier cell line stably up-regulated the clonogenic capacity of miR200c in vitro, with increased migration and invasion ability, and showed the characteristics of multifocal cell lines.