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目的通过2种不同饲料联合链脲佐菌素(STZ)诱导建立代谢综合征(MS)大鼠模型,为该疾病的研究探索理想的建模方法。方法 90只大鼠随机分为3组:MS模型正常盐组(NS)、MS模型高盐组(HS)和对照组(NC)。NS组大鼠饲喂高脂高糖饲料,HS组大鼠饲喂高脂高糖高盐饲料,NC组大鼠饲喂基础饲料。10周末,NS组和HS组选择腹围达标的大鼠,给予一次性腹腔注射STZ25mg/kg。分别在不同时间点测定各组大鼠体重、腹围、血压、空腹血糖及血脂等指标。结果 NS组和HS组大鼠体重、腹围、空腹血糖与NC组相比均升高,差异有统计学意义(P<0.05～0.01)。12周末时,HS组大鼠血糖及腹围较NS组增高,差异有统计学意义(P<0.05);12周末时,HS组大鼠高密度脂蛋白胆固醇较NS组降低,差异有统计学意义(P<0.05)。NS组成模率为40.0%,HS组成模率为66.7%,2组成模率差异有统计学意义(P<0.05)。结论采用高脂高糖饮食或高脂高糖高盐饮食诱导加腹腔注射小剂量STZ的方法可在短时间内成功复制稳定的MS大鼠模型,且HS组成模率较NS组高。
Objective To establish a rat model of metabolic syndrome (MS) induced by two different diets combined with streptozotocin (STZ) and explore the ideal modeling method for the study of this disease. Methods Ninety rats were randomly divided into three groups: normal saline group (NS), MS model high salt group (HS) and control group (NC). Rats in NS group were fed with high-fat and high-sugar diet, rats in HS group were fed with high-fat, high-sugar and high-salt diet, and rats in NC group were fed with basal diet. At the end of 10 weeks, rats in NS group and HS group with abdominal compliance were given a single intraperitoneal injection of STZ25mg / kg. Body weight, abdominal circumference, blood pressure, fasting blood glucose and blood lipids were measured at different time points. Results Compared with NC group, the body weight, abdominal circumference and fasting blood glucose in NS group and HS group were significantly increased (P <0.05 ~ 0.01). At the end of 12th week, the blood glucose and abdominal circumference of rats in HS group were significantly higher than those in NS group (P <0.05). At the end of the 12th week, the levels of HDL-C in HS group were lower than those in NS group Significance (P <0.05). The rate of formation of NS was 40.0% and that of HS was 66.7%. There was a significant difference between the two groups (P <0.05). CONCLUSIONS: Stable MS rat models can be successfully replicated in a short time by inducing high-fat and high-sugar diet or high-fat, high-sugar and high-salt diet plus intraperitoneal injection of low-dose STZ. The HS moduli are higher than those of NS group.