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目的：从小鼠B16黑色素瘤细胞胞浆中提纯热休克蛋白抗原肽复合物(heat shock protein-antigen peptide complexes,HACs)，并观察其抑瘤作用及探索其机制。方法：CNBr-Sepharose 4B亲和层析法提纯B16 HACs，应用体内免疫法检测HAC的抑瘤作用，结晶紫染色法检测γIFN分泌活性，ConA诱导的淋巴母细胞增殖法检测IL2分泌活性，3H-TdR掺入法检测特异性CTL的杀伤活性。结果：亲和层析法获得的HAC60、HAC75和HAC97免疫小鼠后，小鼠移植肿瘤的发生率降低，肿瘤平均出现时间延迟，平均肿瘤重量明显减轻；小鼠脾细胞的γ-IFN和IL-2分泌活性增强，特异性CTL的杀伤活性增强。结论：经亲和层析纯化的HACs可通过增强免疫功能的机制抑制小鼠移植肿瘤的生长，并为制备肿瘤疫苗提供实验依据。
OBJECTIVE: To purify heat shock protein-antigen peptide complexes (HACs) from the cytoplasm of mouse B16 melanoma cells and observe its anti-tumor effect and explore its mechanism. METHODS: B16 HACs were purified by CNBr-Sepharose 4B affinity chromatography. The anti-tumor effect of HAC was detected by in vivo immunoassay. The secretory activity of γIFN was detected by crystal violet staining. The activity of IL2 secretion was detected by ConA-induced lymphoblast proliferation assay. 3H- TdR incorporation was used to determine the killing activity of specific CTLs. RESULTS: After immunized mice with HAC60, HAC75 and HAC97 obtained by affinity chromatography, the incidence of transplanted tumors in mice decreased, the average tumor time was delayed, and the average tumor weight was significantly reduced; γ-IFN and IL in mouse splenocytes -2 increased secretion activity, enhanced killing activity of specific CTLs. CONCLUSION: HACs purified by affinity chromatography can inhibit the growth of transplanted tumors in mice by enhancing the immune mechanism and provide experimental evidence for the preparation of tumor vaccines.