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目的 研究IL 4和IL 10对CD4+T细胞上CXC趋化性细胞因子受体 4(CXCR4)表达的调控。方法 采用流式细胞术 ,实时定量逆转录PCR(RT PCR) ,细胞因子的趋化性分析以及Scatchard分析法检测CXCR4和CXCR4mR NA。结果 IL 4和IL 10能够显著上调或下调CD4+T细胞上CXCR4的表达。SDF 1诱导的CD4+T细胞趋化性同样也受到IL 4和IL 10的调控。IL 4和IL 10能分别上调或下调CD4+T细胞中CXCR4的mRNA表达。在新鲜分离的CD4+T细胞上只存在一种亲和力的CXCR4(Kd6 3nmol·L-1)。而IL 4刺激的CD4+T细胞存在两种不同亲和力的CXCR4(Kd14 4nmol·L-1和Kd2 14 6nmol·L-1)。IL 4和IL 10通过环腺苷酸和环鸟苷酸双重信号途径对CD4+T细胞上CXCR4表达的调控与Ca2 +激活刺激无关。IL 4和IL 10可能是通过CD2 6来调控CXCR4在CD4+T细胞上的表达。结论 IL 4和IL 10对CXCR4 SDF 1受体 配体对的调节 ,在HIV侵袭和炎症感染进程有关的细胞因子环境中起着重要作用
Objective To investigate the regulation of CXC chemokine receptor 4 (CXCR4) expression on CD4 + T cells by IL 4 and IL 10. Methods CXCR4 and CXCR4 mRNA were detected by flow cytometry, real-time quantitative reverse transcription PCR (RT-PCR), chemotaxis analysis of cytokines and Scatchard analysis. Results IL 4 and IL 10 significantly up-regulated or down-regulated CXCR4 expression on CD4 + T cells. SDF 1 -induced CD4 + T cell chemotaxis is also regulated by IL 4 and IL 10. IL4 and IL10 were able to upregulate or down-regulate the mRNA expression of CXCR4 in CD4 + T cells, respectively. There is only one affinity for CXCR4 (Kd6 3 nmol·L-1) on freshly isolated CD4 + T cells. There were two different CXCR4 (Kd14 4 nmol·L-1 and Kd2 146 nmol·L-1) IL-4-stimulated CD4 + T cells. The regulation of CXCR4 expression on CD4 + T cells by both IL-4 and IL-10 via the dual signaling pathway of cyclic adenosine monophosphate and cyclic guanosine is independent of Ca2 + stimulation. It is possible that IL-4 and IL-10 regulate the expression of CXCR4 on CD4 + T cells via CD26. Conclusion The regulation of CXCR4 SDF 1 receptor ligand pair by IL 4 and IL 10 plays an important role in the cytokine environment associated with HIV invasion and inflammatory process progression