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实验研究了使用阳离子表面活性剂Aliquat336与异辛烷构成的反胶团溶液，通过液－液萃取方式纯化工业级α-淀粉酶的过程．当使用的反胶团溶液组成为Aliquat336／isooctane／1％（V／V）n－butanol时，实验发现纯化工业级α-淀粉酶的最佳条件：水相组成为30mmol／L，pH10，或酶，萃取时间为1min；反萃液组成为30mmol／L，pH6，反萃时间为3min在此条件下，通过萃取循环能够达到纯化工业级α-淀粉酶的目的，经过一个萃取与反萃循环后，α-淀粉酶的比活提高1．5倍，酶活回收率达85％与此同时，工业级α-淀粉酶中所含的中性蛋白酶活回收率只有10％，而且其比活降低了近7倍。α-淀粉酶与中性蛋白酶间的分离系数能达10左右．
Experimental study of the use of cationic surfactant Aliquat336 and isooctane reverse micelle solution, by liquid - liquid extraction of industrial-grade α-amylase process. The optimal conditions for the purification of industrial α-amylase were found to be 30mmol / L, pH10, or pH10 when the composition of the reverse micelles was Aliquat336 / isooctane / 1% (V / V) Enzyme, the extraction time is 1min; the composition of the stripping solution is 30mmol / L, pH6, and the stripping time is 3min Under this condition, the purpose of purifying industrial grade alpha-amylase can be achieved through an extraction cycle, and after an extraction and stripping cycle After the α-amylase increased by 1.5 times the specific activity, the recovery rate of 85% enzyme at the same time, the industrial-grade α-amylase contained in the neutral protease activity recovery of only 10%, and its specific activity Reduced by nearly 7 times. α-amylase and neutral protease separation factor of about 10.