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目的:观察小鼠肥大细胞炎症反应在白藜芦醇灌胃减轻Apo E-/-小鼠进展期动脉粥样硬化过程中的作用。方法:30只Apo E-/-小鼠从6周开始,取其中的20只,进行连续24周的高脂饲料喂养,造成进展期动脉粥样硬化模型。于高脂喂养12周后,在20只小鼠中随机分出10只进行白藜芦醇灌胃干预(50 mg/kg)。取心脏主动脉流出道进行石蜡包埋切片,H.E.染色显示大体病变改变,Movat染色显示动脉粥样硬化病变,甲苯胺蓝染色显示结缔组织肥大细胞分布、数量和脱颗粒状态。免疫组织化学显示IL-6、TNF-α表达。结果:经过高脂喂养后,H.E.和Movat染色显示心脏流出道管壁附着粥样硬化斑块面积明显增加,白藜芦醇灌胃12周可以减轻粥样硬化斑块内胆固醇结晶的面积(14.7%vs 10.6%),增加胶原纤维比例(31.9%vs 42.5%),并表现出具有较厚纤维帽的稳定型斑块特征。甲苯胺蓝染色显示在病变组的血管周围结缔组织中,出现成簇的、三五成群的肥大细胞,部分肥大细胞呈现脱颗粒状态。白藜芦醇干预可以减少肥大细胞浸润数量(11.4个/只vs 6.5个/只),抑制脱颗粒反应细胞比例(28.6%vs 11.1%),减少肥大细胞浸润区域IL-6、TNF-α表达。结论:白藜芦醇可能通过小鼠较少血管周围结缔组织中肥大细胞的数量及脱颗粒反应,减轻局部炎症,延缓动脉粥样硬化的发生。
OBJECTIVE: To observe the role of mast cell inflammatory response in mice treated with resveratrol to reduce the progression of atherosclerosis in Apo E - / - mice. Methods: Thirty 30 Apo E - / - mice were fed with high fat diet for 24 consecutive weeks from the 6th week and 20 of them, resulting in a model of advanced atherosclerosis. After 12 weeks of high-fat diet, 10 mice were randomly divided into 20 groups for resveratrol gavage (50 mg / kg). The aortic outflow tract of the heart was paraffin-embedded and stained with H.E., gross lesions were observed. Movat staining showed atherosclerotic lesions. Toluidine blue staining showed connective tissue mast cell distribution, number and degranulation. Immunohistochemistry showed IL-6, TNF-α expression. Results: After high-fat diet, HE and Movat staining showed that the area of atherosclerotic plaque on the wall of cardiac outflow tract was significantly increased. Resveratrol treatment for 12 weeks reduced the area of cholesterol crystals in atherosclerotic plaque (14.7 % vs 10.6%), increased collagen fiber ratio (31.9% vs 42.5%), and showed stable plaque characteristics with a thicker fibrous cap. Toluidine blue staining showed that in the connective tissue around the vessels in the diseased group, there appeared clusters of mast cells in groups of thirty-five, and some of the mast cells showed degranulation. Resveratrol could reduce the number of mast cells infiltration (11.4 / only vs 6.5 / only), inhibit the proportion of degranulation cells (28.6% vs 11.1%) and decrease the expression of IL-6 and TNF-α in mast cells . Conclusion: Resveratrol may reduce the local inflammation and delay the occurrence of atherosclerosis by reducing the number of mast cells and degranulation in connective tissue of mice with less blood vessels.