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为阐明小麦化感抑草的生理机制,选择强化感小麦‘115/青海麦’、‘92L89’和弱化感小麦‘抗10103’,通过添加浓度为0.2%、0.5%和1.0%的小麦根水提液进行水培试验3周后,测定了各处理看麦娘的鲜重,分析叶片中叶绿素(SPAD值)、可溶蛋白、MDA、类黄酮、总酚的含量以及SOD、POD、CAT活性。结果表明,水提液处理显著抑制了看麦娘的生长,抑制率在不同处理浓度及小麦品种间均存在显著差异,强化感小麦的抑制率显著高于弱化感小麦。在处理浓度范围内,不同小麦根水提液的抑制率大小依次为‘115/青海麦’(24.7%~74.3%)>‘92L89’(15.7%~71.6%)>‘抗10103’(13.8%~61.4%);0.2%、1.0%和5.0%水提液处理的抑制率大小依次为13.8%~24.7%、41.7%~66.4%和61.4%~74.2%。看麦娘叶绿素含量(SPAD值)随处理浓度增大显著降低,可溶蛋白含量,SOD、POD、CAT活性,MDA、类黄酮含量随处理浓度增大显著升高,强化感小麦对看麦娘的生理刺激作用高于弱化感小麦。1.0%‘115/青海麦’及5.0%各小麦水提液处理的看麦娘总酚含量高于对照。可见,小麦化感胁迫提高了看麦娘的保护酶系统活性,增强了抗氧化物质代谢,但显著增强了细胞膜脂质过氧化和叶绿素降解,不利于靶标植物看麦娘的生长。
In order to elucidate the physiological mechanism of allelopathic weed resistance in wheat, we selected enhanced wheat ’115 / Qinghai wheat’, ’92L89’ and weakened wheat ’anti 101010’ by adding 0.2%, 0.5% and 1.0% wheat root water Three weeks after the hydroponics, the fresh weights of Anemarrhena asphodeloides were determined. The content of chlorophyll (SPAD), soluble protein, MDA, flavonoids, total phenols and the activities of SOD, POD and CAT . The results showed that the water extract significantly inhibited the growth of A. natans. The inhibition rates were significantly different among different treatments and wheat cultivars. The inhibition rate of wheat with enhanced sensitivity was significantly higher than that of wheat with weakened sensitivity. The inhibitory rates of water extracts of different wheat roots were in the order of ’115 / Qinghai Mai’ (24.7% ~ 74.3%)> 92L89 ’(15.7% ~ 71.6%)>’ 101010 ’(13.8% ~ 61.4%). The inhibitory rates of 0.2%, 1.0% and 5.0% aqueous extracts were 13.8% -24.7%, 41.7% -66.4% and 61.4% -74.2%, respectively. The chlorophyll content (SPAD value) of A. indica decreased significantly with the increase of treatment concentration. The contents of soluble protein, SOD, POD, CAT, MDA and flavonoids increased significantly with the increase of treatment concentration. Physiological stimuli were higher than weakened wheat. 1.0% ’115 / Qinghai Mai’ and 5.0% of the wheat water extract treated Mai Niang total phenol content was higher than the control. It can be seen that the allelopathy of wheat increased the activities of the protective enzyme system and enhanced the metabolism of antioxidants, but significantly enhanced lipid peroxidation and chlorophyll degradation of the cell membrane, which was unfavorable for the growth of the target plant.