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目的 研究短暂全脑缺血后３０ 分开始的低温复合巴曲酶对沙土鼠行为学和组织病理学的影响。方法 采用沙土鼠全脑缺血模型，缺血时间１０ 分。动物随机分为５组：假手术组、常温组、延迟性低温组、巴曲酶组、延迟性低温复合巴曲酶组，每组７只。低温（３２℃）于再灌注３０分给予，维持３小时；巴曲酶８ＢＵ·ｋｇ－ １再灌注３０ 分经腹腔注入。于动物存活第５ 天行开阔法行为学检查，第７ 天行海马ＣＡ１区组织病理学检查。结果 行为学结果显示，常温组沙土鼠的探索活动明显较假手术组活跃；巴曲酶组或延迟性低温组沙土鼠的探索活动均较常温组明显减弱，较延迟性低温复合巴曲酶组明显活跃。组织病理学结果显示，常温组海马ＣＡ１ 区内侧、中间和外侧存活神经元计数均较常温组明显增多；延迟性低温组或巴曲酶组海马ＣＡ１ 区内侧、中间和外侧存活神经元计数均较延迟性低温复合巴曲酶组明显减少。结论 延迟性低温或巴曲酶可以减轻脑缺血后神经功能障碍和海马神经元坏死，两者复合的作用更强。
Objective To investigate the effects of low temperature compound batroxobin on behavior and histopathology of gerbils starting from 30 minutes after transient global cerebral ischemia. Methods The gerbil model of global cerebral ischemia was used. The ischemic time was 10 min. The animals were randomly divided into 5 groups: sham operation group, normal temperature group, delayed hypothermia group, batroxobin group, delayed hypothermia complex batroxobin group, 7 rats in each group. Low temperature (32 ℃) in the reperfusion for 30 minutes to give, maintain 3 hours; Batroxobin 8BU · kg-1 reperfusion 30 minutes by intraperitoneal injection. On the fifth day after the animal survived, the behavioral examination was conducted. The hippocampal CA1 region histopathological examination was performed on the seventh day. Results The results of behavioral study showed that the exploratory activities of gerbils in normal temperature group were significantly more active than those in sham operation group. The exploratory activities of gerbils in Batroxobin group and delayed hypothermia group were significantly weaker than those in normal temperature group. Compared with delayed hypothermia complex batroxobin group, Obviously active. The results of histopathology showed that the number of surviving neurons in the medial, medial and lateral hippocampal CA1 areas were significantly increased compared with the normal temperature group in normal temperature group. The neurons in medial, medial and lateral areas of hippocampal CA1 area in delayed hypothermia group or batroxobin group were more than those in normal temperature group Delayed low-temperature compound batroxobin group significantly reduced. Conclusion Delayed hypothermia or batroxobin can alleviate the neurological dysfunction after cerebral ischemia and hippocampal neuron necrosis, the combination of the role of both stronger.