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合成了两种新型三齿多吡啶钴(Ⅱ)和钌(Ⅱ)的混配配合物[Co(TolylTPy)(H2Bzimpy)]Cl2[TolylTPy=4’-对甲基苯基-2,2’:6’,2’’-三联吡啶,H2Bzimpy=2,6-二(苯并咪唑-2)吡啶](A)和Ru(TolylTPy)(Bzimpy)(B).用元素分析,IR,1HNMR等对它们进行了表征,测定了配合物B的晶体结构,用电子吸收光谱、荧光光谱等研究了配合物与小牛胸腺DNA(CTDNA)的相互作用及其对pBR322DNA的断裂作用.结果表明,配合物A和B与CTDNA的作用属静电结合,凝胶电泳实验说明配合物A在310nm光辐射15min,可使超螺旋pBR322DNA断裂为开环缺口型和线型DNA.
Two novel complexes of cobalt (Ⅱ) and ruthenium (Ⅱ) [Co (TolylTPy) (H2Bzimpy)] Cl2 [TolylTPy = 4’-p-methylphenyl- H2Bzimpy = 2,6-bis (benzimidazole-2) pyridine] (A) and Ru (TolylTPy) (Bzimpy) (B) were determined by elemental analysis, IR, The crystal structure of complex B was determined and the interaction between the complex and calf thymus DNA (CTDNA) and its cleavage of pBR322 DNA were studied by electron absorption spectroscopy and fluorescence spectroscopy.The results showed that the complex The interaction of A and B with CTDNA belongs to electrostatic binding. The results of gel electrophoresis showed that the complex A was exposed to 310 nm light for 15 min, and the supercoiled pBR322 DNA could be cleaved into open-loop gap type and linear DNA.