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目的:检测YAP和Survivin的表达水平,探讨YAP及Survivin表达与胃癌发生的关系和意义。方法:采用PV9000免疫组化方法分别检测98例胃癌及配对正常胃黏膜,58例肠上皮化生,32例不典型增生组织中YAP和Survivin的表达水平。结果:YAP在不典型增生(37.5%)、胃癌组织(48.0%)中的阳性表达率显著高于正常胃黏膜组织(13.3%),P<0.01;Survivin在肠上皮化生(53.4%)、不典型增生(59.4%)及胃癌(65.3%)组织中的阳性表达率显著高于正常胃黏膜组织(11.2%),P<0.01;弥漫型胃癌组(74.6%)Survivin阳性表达率显著高于肠型胃癌组(51.3%),P<0.05。伴淋巴结转移胃癌组Survivin阳性表达率(76.9%)显著高于无淋巴结转移组(41.2%),P<0.01。在98例胃癌组织中YAP与Sur-vivin表达呈正相关,等级相关系数rk=0.246,P<0.01。结论:YAP可能诱导凋亡抑制因子Survivin的表达,Survivin可能通过抑制胃癌中细胞凋亡及调节细胞有丝分裂的发生进而参与胃癌的发生、发展及转移。将两指标联合检测,有助于胃癌的早期诊断及对预后的评估。
Objective: To detect the expression of YAP and Survivin and to explore the relationship between the expression of YAP and Survivin and the occurrence of gastric cancer. Methods: The expression of YAP and Survivin in 98 cases of gastric cancer, paired normal gastric mucosa, 58 cases of intestinal metaplasia and 32 cases of atypical hyperplasia were detected by PV9000 immunohistochemical method. Results: The positive expression rate of YAP in atypical hyperplasia (37.5%) and gastric carcinoma (48.0%) was significantly higher than that in normal gastric mucosa (13.3%), P <0.01. Survivin was found in intestinal metaplasia (53.4% The positive rate of Survivin expression in atypical hyperplasia (59.4%) and gastric cancer (65.3%) was significantly higher than that in normal gastric mucosa (11.2%) (P <0.01) Intestinal gastric cancer group (51.3%), P <0.05. The positive rate of Survivin expression in gastric cancer with lymph node metastasis (76.9%) was significantly higher than that in non-lymph node metastasis (41.2%), P <0.01. There was a positive correlation between the expression of Survivin and YAP in 98 cases of gastric cancer, the rank correlation coefficient rk = 0.246, P <0.01. Conclusion: YAP may induce the expression of Survivin, Survivin may participate in the occurrence, development and metastasis of gastric cancer by inhibiting apoptosis and regulating cell mitosis in gastric cancer. Joint detection of the two indicators, contribute to the early diagnosis of gastric cancer and prognosis.