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目的 建立α粒子辐射诱导人肺鳞癌裸鼠转移模型。方法 α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种裸鼠皮下 ,后采用组织块接种的方法 ,在鼠间连续传代 3次 ,计算原代成瘤率及传代成活率 ,并观察肿瘤的转移情况 ,病理切片和免疫组织化学鉴定肿瘤的性质和来源。结果 (1)α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种成瘤率为 4 4 4 % (4 9) ;(2 )原代肿瘤的体表转移率与肺转移率均为 2 5 % (1 4 ) ;(3)病理切片鉴定为低分化鳞状上皮细胞癌 ;(4)免疫组织化学显示CK3和EMA表达阳性。结论 采用组织块接种的方法 ,在α粒子辐射诱导人肺鳞癌细胞系BERP35T4的基础上 ,经过裸鼠体内连续传代 3次 ,成功地建立了人类肺癌裸鼠转移模型。
Objective To establish a metastatic model of human lung squamous carcinoma in nude mice induced by α particle irradiation. METHODS: Human lung squamous cell carcinoma cell line BERP35T4 was inoculated subcutaneously in nude mice by means of α-particle irradiation. The tumor cells were inoculated three times in succession, and the primary tumorigenicity and passage survival rate were calculated. Metastasis, biopsy and immunohistochemistry to identify the nature and origin of the tumor. Results (1) The primary tumorigenic rate of human lung squamous cell carcinoma cell line BERP35T4 induced by α-particle irradiation was 44.4% (49). (2) The rates of surface metastasis and lung metastasis of primary tumor were both 25 % (14); (3) pathological section was identified as poorly differentiated squamous cell carcinoma; (4) Immunohistochemistry showed positive CK3 and EMA expression. CONCLUSION: The human lung cancer cell line BERP35T4 was induced by tissue particle inoculation method. The nude mouse lung metastasis model was established successfully by three consecutive passages in nude mice.