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据报道64DP是一种与肿瘤相关的人血清DNA结合蛋白质,其现有的定量测定方法略嫌繁琐粗糙。本文采用亲和层析法纯化的兔抗64DP抗体,建立了简便灵敏的酶免疫测定法(ELISA)。部分样本经ELISA和火箭电泳两种方法平行测定,证明两者有良好的相关性。对70例恶性肿瘤及86例正常对照血清的测定结果,肿瘤组平均64DP水平显著高于对照组,其中尤以肝癌、胃癌及肺癌的增高更为显著。对非肿瘤其它疾病患者的检测发现感染和外伤也能造成血清64DP的迅速增加。本研究表明血清64DP的改变不仅仅局限于恶性肿瘤,它具有急性期反应蛋白质的特征。这使64DP作为肿瘤标志物应用于临床受到一定限制。
It has been reported that 64DP is a tumor-associated human serum DNA-binding protein, and its existing quantitative assay method is slightly tedious and rough. In this study, rabbit anti-64DP antibody purified by affinity chromatography was used to establish a simple and sensitive enzyme immunoassay (ELISA). Some of the samples were assayed by ELISA and rocket electrophoresis in parallel, which proved that the two have good correlation. The results of the measurement of 70 cases of malignant tumors and 86 cases of normal control serum showed that the average 64DP level in the tumor group was significantly higher than that in the control group, and the increase in liver cancer, gastric cancer, and lung cancer was particularly significant. Detection of patients with non-neoplastic other diseases found that infection and trauma can also cause a rapid increase in serum 64DP. This study shows that the change of serum 64DP is not limited to malignant tumors, it has the characteristics of acute phase response proteins. This makes 64DP as a tumor marker applied to the clinical subject to certain limitations.