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茶碱、头孢唑啉、头孢拉定、头孢哌酮均为临床常用的治疗药物,且茶碱与另外3种抗生素联用的机会很大.4种药物的HPLC法血药浓度测定国内虽各有报道,但作者未见有同时测定的报道.1 仪器与试剂1.1仪器HPLC色谱仪:200—LC泵、LC—295紫外检测仪、1022型积分仪(美国PE公司).1.2 试剂 水杨酸(北京化工厂,AR);茶碱(卫生部临床检验中心);头孢唑啉钠针(齐鲁制药厂,批号9505124);头孢拉定(上海施贵宝制药有限公司,批号960604);头孢哌酮钠(广州制药厂,批号960309);甲醇(色谱纯,天津四友公司);醋酸、醋酸安和高氯酸均为分析纯.
Theophylline, cefazolin, cefradine, cefoperazone are commonly used in clinical treatment of drugs, and theophylline with three other antibiotics opportunities are great .4 kinds of drugs by HPLC method for the determination of plasma concentrations have been reported in the country , But the authors did not see the simultaneous determination of reports.1 Instruments and reagents 1.1 Instrument HPLC Chromatograph: 200-LC pump, LC-295 UV detector, 1022 integrator (PE) 1.2 reagent salicylic acid (Beijing Chemical plant, AR); theophylline (Ministry of Health clinical test center); sodium cefazolin needle (Qilu Pharmaceutical Factory, batch number 9505124); cefradine (Shanghai Shi Guibao Pharmaceutical Co., Ltd., batch number 960604); cefoperazone sodium (Guangzhou Pharmaceutical Factory, ); Methanol (pure chromatography, Tianjin Friends of the four companies); acetic acid, acetic acid and perchloric acid were of analytical grade.