细胞凝块经固定脱水制成的细胞学蜡块可用于细胞涂片比对、免疫组化及分子学检测,已成为细胞学诊断的一项重要途径~([1-3])。细胞凝块的制作需要考虑2个条件:1保证细胞微细结构完好性;2尽量缩短细胞的凝集时间;这样可使细胞学蜡块真正成为细胞学的常规制作方法。4%中性缓冲甲醛、95%乙醇均为细胞常用的固定剂,因其固定原理不同,对细胞的固定状态亦有差异。我们在工作中一般采用95%乙醇制作细胞凝块,或先用4%中性甲醛固定15 min后 Cytoskeletal paraffin block made by fixed dehydration can be used for cell smear comparison, immunohistochemistry and molecular detection, cytology has become an important way to ~ ([1-3]). Cell clot production need to consider two conditions: 1 to ensure the fine structure of the cell integrity; 2 try to shorten the cell aggregation time; This can make the cytology wax block truly become a routine method of making cytology. 4% neutral buffered formaldehyde, 95% ethanol are commonly used cell fixing agent, because of its fixed principle, the fixed state of the cells are also different. We work in the general use of 95% ethanol cell clotting, or fixed with 4% formaldehyde for 15 min