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法国小麦和中国小麦杂交的后代和鸭茅状摩擦禾(父本)杂交,授粉1 d后用100 mg/L 2,4-D蘸穗处理,12~14 d取单倍体幼胚培养。结果表明,仅培养于1/2 MS培养基上,成苗率为78.01%;而根据幼胚的发育状态,先将发育较完善的幼胚直接接种在1/2 MS培养基上,将发育不良的幼胚先接种于1/2 MS+2 mg/L 2,4-D的培养基上进一步养育幼胚,3~4 d后再将其转入1/2 MS培养基中,此法的成苗率为93.22%,较前者的成苗率提高了15.21个百分点。
The offsprings of French wheat and Chinese wheat were crossed with Rubia cordifolia (Paternal), pollinated for 1 day with 100 mg / L 2,4-D dip, and harvested for 12-14 days. The results showed that the seedling rate was only 78.01% when cultured on ½ MS medium only. On the basis of the developmental status of immature embryos, the immature embryos with better development were directly inoculated on 1/2 MS medium, Immature immature embryos inoculated first in 1/2 MS +2 mg / L 2,4-D medium further raising immature embryos, 3 ~ 4 d and then transferred to 1/2 MS medium, this method The seedling rate was 93.22%, which was 15.21% higher than the former.