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目的对八氯代二苯并二噁英(1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin,OCDD)暴露对小鼠胚胎发育的毒性效应做分析和评价。方法选取性成熟昆明小鼠120只,其中雌鼠80只,雄鼠40只,以雌∶雄比例2∶1配种使雌鼠妊娠。将妊娠雌鼠随机分为4组,每组15只,分别以OCDD低(1.00μg/ml)、中(10.0μg/ml)、高(100μg/ml)剂量灌胃染毒,以药物溶剂处理做空白对照组,各小鼠灌胃溶液体系均为10 ml/kg。每日晨灌胃1次,连续10 d。对各组雌鼠增重情况、胚胎生长发育情况及致畸率进行比较。结果雌鼠体重增长率最高的对照组,其吸收胎、死胎率仅为1.32%,致畸率仅为0.67%,四组雌鼠的增重情况比较存在差异,中剂量实验组和高剂量实验组吸收、死胎率和致畸率均在25%以上,较空白对照组吸收、死胎率差异有统计学意义(P<0.05)。结论八氯代二苯并二噁英暴露对小鼠胚胎发育的毒性效应明确,其增加小鼠胚胎的吸收、死亡以及致畸。
Objective To analyze and evaluate the toxic effects of exposure to octachlorodibenzo-p-dioxin (OCDD) on embryonic development in mice. Methods Totally 120 Kunming mice were selected, including 80 females and 40 males. Pregnant females were dosed with female to male ratio 2: 1. The pregnant female rats were randomly divided into 4 groups with 15 rats in each group. The mice were orally administered with low OCDD (1.00μg / ml), medium (10.0μg / ml) and high (100μg / ml) Make a blank control group, each mouse gavage solution system is 10 ml / kg. Morning gavage 1 times a day for 10 days. The weight gain of each group of females, embryonic growth and development and teratogenicity were compared. Results In the control group with the highest rate of body weight gain, the rate of fetus fetus stillbirth was only 1.32% and the rate of teratogenic rate was only 0.67%. The weight gain of the four groups of females was different. The middle dose group and high dose group The absorption rate, stillbirth rate and teratogenic rate were all higher than 25%, which were significantly higher than those of the blank control group (P <0.05). Conclusions The toxic effect of octaclor-dioxin exposure on mouse embryo development is clear, which increases the absorption, death and teratogenicity of mouse embryos.