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在“高油115”玉米幼苗地下部施用4种不同浓度(10、50、100、200μmol.L-1)的茉莉酸3～48h后,采用生化成分分析和基因表达分析方法,检测了处理部位(根系)和非处理部位(叶片)中防御物质及防御相关基因的表达,以探讨茉莉酸诱导玉米地下部对化学防御反应影响的时间和浓度效应.结果表明:茉莉酸处理玉米地下部对处理部位(根系)和非处理部位(叶片)化学防御反应的影响均与茉莉酸作用的时间和浓度相关.茉莉酸处理玉米地下部后,在3～12h内就能直接诱导处理部位(根系)Bx9、PAL、PR-2a、MPI和FPS基因的表达,使得根中的丁布含量增加而总酚含量下降,其中100μmol.L-1茉莉酸处理浓度产生的诱导作用最强,其次是50μmol.L-1,再次是10μmol·L-1;后期诱导作用则逐渐减弱.另外,茉莉酸处理玉米地下部还能间接影响到非处理部位(叶片)的化学防御反应,50μmol·L-1茉莉酸在处理后3h就能系统诱导叶片中Bx9和FPS基因的表达,使叶片的丁布含量增加;在6～24h内则使叶片Bx9、PAL、PR-1、MPI和TPS基因表达量增加,丁布和总酚含量降低.可见,对于大部分检测指标来说,茉莉酸处理玉米地下部对地上部的间接诱导作用不如对地下部的直接诱导作用强,根系启动防御反应的时间较叶片早,同时,随着处理浓度的增加,茉莉酸对根系和叶片防御反应的诱导作用有增强趋势.
Four different concentrations of jasmonic acid (10, 50, 100, 200μmol.L-1) were applied to the underground of “Gaoyou115 ” maize seedlings for 3 ~ 48h. After biochemical analysis and gene expression analysis (Roots) and non-treatment sites (leaves) in order to explore the time and concentration effects of jasmonic acid on the chemical defense response induced by jasmonic underground.Results showed that jasmonic acid treatment of corn underground The effect of chemical defense reaction on treatment site (root) and non-treatment site (leaf) was related to the time and concentration of jasmonic acid action.JA treatment of corn underground could induce the treatment site directly within 3 ~ 12h ), The expression of Bx9, PAL, PR-2a, MPI and FPS genes increased the content of butyricin and the total phenolic content in roots, of which 100μmol.L-1 jasmonic acid had the highest inducing effect, followed by 50μmol .L-1, again 10μmol·L-1; late induction gradually weakened.In addition, jasmonic acid treatment of corn underground can indirectly affect the non-treated parts (leaf) chemical defensive response, 50μmol·L-1 jasmine Acid 3h after treatment system Induced the expression of Bx9 and FPS genes in leaves, and increased the content of butyricin. Within 6 ~ 24h, the expression of Bx9, PAL, PR-1, MPI and TPS genes increased and the content of butyricin and total phenols decreased. It can be seen that for most of the test indicators, the indirect induction of ground parts by corn jasmonic acid treatment was not as strong as the direct induction of ground parts, and the root defense reaction started earlier than the leaves. At the same time, Increased, jasmonic acid on the root and leaf defense response induced role has an increasing trend.