目的　了解近 3年泌尿系感染常见致病菌菌群变迁及耐药性变化。方法　将 5 34例中段尿培养阳性者按年份分 3组进行菌群分析 ,和细菌对常用抗生素耐药性对比研究。结果　G-菌所致泌尿系感染率为 76 5 4 %、6 9 90 %和 6 1 70 %(P <0 0 1) ,G+ 菌分别为 16 0 5 %、19 3%和 2 5 % (P <0 0 5 ) ,真菌分别为 7 4 1% ,10 8%和 13 3% (P <0 0 5 )。G+ 菌对常用抗生素的耐药率在 2 0 %～ 80 %之间 ,且随年份而上升。G-菌对丁胺卡那和头孢他啶 ,G+ 菌对万古霉素和呋喃妥因的敏感性较高。结论　G+ 菌及真菌感染有上升趋势。常用抗生素的敏感性下降 ,耐药现象严重 ,应引起临床医生高度重视 Objective To understand the changes of bacterial pathogens and drug resistance of common pathogens in urinary tract infection in recent 3 years. Methods A total of 5 34 patients with positive urinary culture were divided into 3 groups according to the year to conduct the flora analysis and the comparative study on bacterial resistance to common antibiotics. Results The urinary tract infections caused by G- bacteria were 76.54%, 69.9% and 61.7%, respectively (P <0.01), G + bacteria were 160%, 19.3% and 25% P <0 05). The fungi were 741%, 108% and 133% respectively (P <0.05). The resistance rates of G + bacteria to commonly used antibiotics ranged from 20% to 80% and increased with age. G-bacteria for amikacin and ceftazidime, G + bacteria higher susceptibility to vancomycin and nitrofurantoin. Conclusion G + bacteria and fungal infections are on the rise. Commonly used antibiotics decreased sensitivity, drug resistance is serious, should cause clinicians to attach great importance