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目的:探讨人巨细胞病毒感染与男性不育的关系。方法:分析85例男性不育患者病毒感染状况,采用核酸扩增PCR荧光定量技术进行病毒检测,计算机自动分析精子活率和活力,精子形态检测系统下人工修正方法进行精子形态分析。结果:85例男性不育精液样本中人巨细胞病毒阳性率3.8%,与15例生育组比较有统计学差异(P<0.05),病毒感染阳性组精子密度、精液pH值、精液量、液化时间、形态正常精子百分率与病毒感染阴性组比较无显著性差异(P>0.05)。病毒感染阳性组精子的头部畸形率显著高于病毒感染阴性组(P<0.05)。结论:人巨细胞病毒感染可能导致精子头部畸形,人巨细胞病毒感染与男性不育间可能存在联系。
Objective: To investigate the relationship between human cytomegalovirus infection and male infertility. Methods: The virus infection in 85 male infertility patients was analyzed. The virus was detected by PCR amplification of nucleic acid amplification. The sperm motility and viability were analyzed automatically by computer, and the sperm morphological analysis was performed by artificial modification method of sperm morphology detection system. Results: The positive rate of human cytomegalovirus in 85 samples of male infertility was 3.8%, which was significantly different from that of 15 cases of fertility (P <0.05). The positive rate of sperm concentration, sperm pH, sperm volume, liquefaction There was no significant difference in time, morphological normal sperm percentage and negative viral infection group (P> 0.05). The head deformity of the positive group was significantly higher than that of the negative group (P <0.05). Conclusion: Human cytomegalovirus infection may lead to sperm head deformity. Human cytomegalovirus infection may be associated with male infertility.